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A new development in virus inactivation with natural ingredients (catechin EGCg ) !

Green tea catechin EGCg, which is expected to have an antiviral effect as a natural ingredient, has been verified and announced as having an inactivating effect against viruses not only in Japan but also overseas.

Furthermore, it exhibited the effect of preventing invasion into cells by binding to viral proteins (such as spikes) against various viruses that repeatedly mutate, such as influenza and new coronaviruses.

As described above, it was confirmed in the verification experiment by the university research team that this aqueous solution has the effect of inactivating viruses.

Prevention of tooth decay and bad breath

The cause of tooth decay is Streptococcus mutans, which attaches to the teeth and creates acid that dissolves the enamel on the surface of the teeth, resulting in tooth decay. Catechin suppresses the growth of Streptococcus mutans, so it can prevent tooth decay.

Antibacterial action

Catechin has an antibacterial effect, so it suppresses the growth of food poisoning bacteria such as O-157 (enterohemorrhagic Escherichia coli), which causes food poisoning, and Helicobacter pylori, which causes stomach ulcers and stomach cancer.

●Antioxidant ●Anticancer ●Cholesterol lowering

●Prevention of blood sugar rise ●Prevention of obesity, etc.

current challenges

Polyphenols including catechins are very unstable because they are easily oxidized by oxygen dissolved in water or oxygen in the air.

When dissolved in an aqueous solvent, the expected useful action such as the action of removing active oxygen is reduced, and the storage stability is poor, such as discoloration due to oxidation.

As a result, it is difficult to dissolve it in an aqueous solvent and handle it in a liquid state, which is a major obstacle in applying it to foods, beverages, cosmetics, pharmaceuticals, and the like.

New development for problem solving

■ Know the weaknesses of catechins

<Because of its strong antioxidant power>

   ↓

1. In an aqueous solution, it is difficult to stabilize because it is easily oxidized by combining with dissolved oxygen.

2. It is difficult to incorporate into the body because it is common to derivatize using chemical substances for stabilization.

Technology of stabilized high-concentration catechin (EGCg) aqueous solution

Catechin EGCg, which has a strong antioxidant effect, undergoes severe oxidative deterioration and lacks storage stability, which has been a major drawback in the development of various products.

However, this technology is the world's first processing technology that stabilizes it in an aqueous solution at a high concentration.

At present, this aqueous solution formulation is stabilized at a high concentration of 10,000 ppm, and the concentration can be easily adjusted by dilution.

In addition, since all of the ingredients that make it stable are food additive ingredients that are approved by the Ministry of Health, Labor and Welfare, there is no need to worry about side effects even if they enter the body, and oral care, etc.

In addition, although it depends on the concentration of the additive, if the concentration shows the virus inactivation effect, there is almost no damage to the original taste, aroma, and color of existing products due to the astringency, bitterness, and discoloration peculiar to catechin EGCg. products in various fields.

[Characteristics of high-concentration catechin (EGCg)-containing aqueous solution]

● Even if time passes (about 9 months), the survival rate is 72%, and most of

　 them do not deteriorate.

● Since it is stabilized at a high concentration of 10,000ppm, it is easy to dilute.

● Made only with ingredients approved as food additives by the Ministry of Health,

     Labor and Welfare

● At the concentration at which it is effective, it is tasteless, odorless, colorless,

     and transparent, so it has excellent versatility.

Image of deactivation effect

Viruses have protrusions (spikes) in their structures, and there are joints that attach to cells via these, and the spikes are unique structures for each virus. For this reason, prevention by means of a vaccine is to create antibodies against the virus in the body, which then capture specific structures on the spikes and prevent the virus from attaching to cells.

Because of this mechanism, for example, there are multiple influenza viruses with different spike structures, and if the antibody produced by the vaccine does not bind to the specific structure of the spike, influenza virus infection cannot be prevented. There is a problem.

On the other hand, the antiviral effect of catechin, that is, the effect of preventing virus infection to the human body, etc., is that catechin EGCg covers the joints that attach to cells regardless of the difference in the specific structure of various virus spikes.

◆ Anti-influenza virus effect of EGCg

For influenza viruses A and B, EGCg inhibited the adsorption and entry of influenza viruses into cells at concentrations significantly lower than the cytotoxic concentration. Direct contact between virus particles and EGCg is effective in inhibiting virus infection.

◆Summary of inactivation of HCoV-229E by EGCg

It was revealed that EGCg reduces the infectivity of HCoV-229E through two actions: direct inactivation and adsorption inhibition.

In addition, its EC50 was about 0.5 ppm to 2 ppm, suggesting that it exhibits antiviral effects at very low concentrations.

The developed catechin EGCg aqueous solution exhibits antiviral effects by inhibiting the adsorption and entry of influenza viruses and coronaviruses into host cells.

Antiviral activity of catechin EGCg aqueous solution was also confirmed in influenza virus infection system using mice.

Direct contact between EGCg and virus particles is important.

① As a catechin EGCg spray, it is sprayed on the entire face such as the

     throat and nasal cavity, and the effect is achieved by direct contact with

     the mucous membrane.

② Its action and effect as an antibacterial spray that does not use alcohol.

③ Effects of directly adsorbing catechin EGCg into the oral cavity with

     tablets, etc.

④ Effects of catechin EGCg by directly spraying it not only on humans but

     also on livestock, etc., or mixing it with water and giving it to drink.

     *As a disinfectant that is friendly to both livestock and the environment.

⑤ As a health supplement for pets.

⑥ As a health drink by adding to various alcoholic beverages and beverages.